Antinuclear Antibodies (ANA) with Reflex

dsDNA, Connective Tissue Disease

Antinuclear antibodies (ANA) are specific serological markers for the diagnosis and disease management of patients with connective tissue diseases (CTD). Smith antibodies are highly specific, although insensitive, clinical markers for systemic lupus erythematosus (SLE). U1-RNP antibodies are used to diagnosis SLE and mixed connective tissue disease (MCTD). RNP 70 antibodies are more specific for MCTD. U1RNP antibodies in the absence of RNP 70 may indicate SLE. SS-A/Ro antibodies can be a marker for SLE and Sjögren’s syndrome. SS-B/La antibodies are highly specific clinical markers for Sjögren’s syndrome, but a small proportion of patients can remain SS-B/La negative. CENP antibodies are found in patients with CREST Syndrome. Scl-70 antibodies are characteristic and specific for scleroderma. Jo-1 antibodies can be found as markers in polymyositis, dermatomyositis and intestinal pneumonitis. Assay results should be used in conjunction with clinical findings and other serological tests. In accordance with the ACR classification, the EliA ANA screen correlates with alternative methods for detection and contains these antigens; U1RNP (RNP 70, A, C), SSA/Ro (60 kDa, 52 kDa), SSB/La, Centromere B, Scl-70, Jo-1 proteins, and native purified Sm proteins.

Serum

Tiger

SST

Allow serum tubes to clot for a minimum of 30 minutes before centrifuging. Centrifuge within 1 hour of collection and no longer than 2 hours after collection. Failure to separate red cells from serum with 2 hours of collection may lead to inaccurate results.

2 mL

Caution: Serum samples are not to be repeatedly frozen and thawed, since this can cause analyte deterioration. They are to be thawed only once.

Stored at 22˚C: 8 hrs
Stored at 2-8˚C: 2 weeks
 If assays are not completed within 2 weeks or the specimen is to be stored beyond 2 weeks, the serum should be frozen at or below -20˚C.

Plasma, lipemic, hemolyzed or microbially contaminated samples.

Fluorescent enzyme immunoassay (FEIA)

2-5 days

Monday-Friday, alternating days

ANA Screen
dsDNA IgG

86038
86225

If Antinuclear Antibodies (ANA) is positive, an ENA Reflex Panel will be performed. The ENA Reflex Panel includes: SmDp-S IgG, U1RNP IgG, RNP 70 IgG, Scl-70s IgG, Jo-1 IgG, CENPB IgG, SSA/Ro IgG, and SSB/La IgG.

Incyte Diagnostics

5047-6