Laboratory Services

  1. Laboratory Services
  2. Test Directory

Antinuclear Antibodies, HEp-2 Substrate, IgG, Serum

Print this page

Updated Test Information:

Test Description
Antinuclear Antibodies, HEp-2 Substrate, IgG, Serum
Synonym(s)

ANA (Antinuclear Antibodies); Antinuclear Antibodies (ANA), HEp-2 substrate, Serum; LE (Lupus Erythematosus) Prep; Antinuclear Antibody, Serum; ANA IFA; ANA IF
Test ID
NAIFA
General Information

Useful for evaluating patients suspected of having systemic rheumatic disease
Specimen Type

Serum
Specimen Requirements

  • Serum Gel (SST)

  • Red Top
Minimum Sample Volume

0.3 mL
Stability

  • Refrigerated (preferred): 21 days

  • Frozen: 28 days
Limitations

Some patients without clinical evidence of an autoimmune disease or a systemic rheumatic disease may have a detectable level of antinuclear antibody (ANA). This finding is more common in women than men and the frequency of a detectable ANA in healthy women over 40 years of age may approach 15% to 20%. ANA may also be detectable following viral illnesses, in chronic infections, or in patients treated with many different medications.
Methodology

Indirect Immunofluorescence
Estimated TAT

1-2 days
Testing Schedule

Monday-Saturday
Test Includes

  • Antinuclear Ab, HEp-2 Substrate

  • ANA Titer

  • ANA Pattern

  • ANA Titer 2

  • ANA Pattern 2

  • Cytoplasmic Pattern
Retention

14 days
CPT Code(s)

86039
Reference Range

<1:80 (Negative)
Performing Lab

Mayo
LOINC Code(s)

59069-5, 33253-6, 49311-4, 33253-6, 49311-4, 55171-3, 77202-0
Additional Information