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HLA-B27, Blood

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Updated Test Information:

Test Description
HLA-B27, Blood
Synonym(s)

B27; Histocompatibility Antigen; Human Leukocyte Antigen; Leukocyte Antigens; LY27; Spondylitis; W-27 (HLA-B27)
Test ID
LY27B
General Information

Useful for diagnosis of ankylosing spondylitis, juvenile rheumatoid arthritis, and Reiter syndrome
Specimen Type

Whole Blood
Specimen Requirements

Lavender top (EDTA)
Specimen Collection / Processing Instructions

Send whole blood specimen in original tube. DO NOT ALIQUOT.
Minimum Sample Volume

1 mL
Additional Processing Details

Specimen must arrive within 96 hours of draw. Collect Monday-Thursday only.
Stability

Ambient: 4 days
Unacceptable Specimen Conditions

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.
Limitations

  • Orders received for both this test and SSO1 / HLA Class I Molecular Phenotype, Blood or DISI / HLA Class I Molecular Typing Disease Association (which provides data on all HLA Class I low-resolution antigens, including B27) will be questioned due to test overlap. This HLA-B27 test is best used alone if a particular disease such as ankylosing spondylitis is under consideration

  • Extreme temperature changes during shipping may alter the specimen making it unacceptable for testing
Methodology

Flow Cytometry
Estimated TAT

2-6 days
Testing Schedule

Monday-Saturday
Retention

7 days
CPT Code(s)

86812
Reference Range

An interpretive report will be provided
Performing Lab

Mayo
LOINC Code(s)

26028-1, 69052-9
Additional Information