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HLA-B27, Blood

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Updated Test Information:

Test Description
HLA-B27, Blood
Synonym(s)

B27; Histocompatibility Antigen; Human Leukocyte Antigen; Leukocyte Antigens; LY27; Spondylitis; W-27 (HLA-B27)
Test ID
LY27B
Performing Lab

Mayo
General Information

Useful for diagnosis of ankylosing spondylitis, juvenile rheumatoid arthritis, and Reiter syndrome
Specimen Type

Whole Blood
Specimen Requirements

Lavender top (EDTA)
Specimen Collection / Processing Instructions

Send whole blood specimen in original tube. DO NOT ALIQUOT.
Minimum Sample Volume

1 mL
Additional Processing Details

Specimen must arrive within 96 hours of draw. Collect Monday-Thursday only.
Stability

Ambient: 4 days
Unacceptable Specimen Conditions

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.
Limitations

  • Orders received for both this test and SSO1 / HLA Class I Molecular Phenotype, Blood or DISI / HLA Class I Molecular Typing Disease Association (which provides data on all HLA Class I low-resolution antigens, including B27) will be questioned due to test overlap. This HLA-B27 test is best used alone if a particular disease such as ankylosing spondylitis is under consideration

  • Extreme temperature changes during shipping may alter the specimen making it unacceptable for testing
Methodology

Flow Cytometry
Estimated TAT

2-6 days
Testing Schedule

Monday-Saturday
Retention

7 days
CPT Code(s)

86812
Reference Range

An interpretive report will be provided
LOINC Code(s)

26028-1, 69052-9
Additional Information