Laboratory Services

Protime, INR and Activated Partial Thrombin Time

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Updated Test Information:

Test Description
Protime, INR and Activated Partial Thrombin Time
Synonym(s)

PT, INR, PTT, APTT

Test ID
PTPTT
Specimen Type

Plasma

Specimen Requirements

Light Blue
Aliquot Tube

Specimen Collection / Processing Instructions

Platelet poor Coag Citrate Specimen processing:
1. Gently invert tube 2-3 times prior to placing in Centrifuge
2. Centrifuge Citrate whole blood at 4500 rpm for 10 minutes
3. Carefully pipette plasma, avoiding the platelet/buffy coat into a plastic vial
4. Centrifuge plasma at 4500 rpm for 10 minutes
5. Pipette the plasma to a plastic vial, leaving the bottom 0.25 mL undisturbed
6. Outside locations freeze plasma immediately and transport frozen. Samples processed inhouse, if technician can run immediately, you do not need to freeze, if not freeze immediately.

Minimum Sample Volume

The sodium citrate collection tube must be filled to 90% of capacity.

Pediatric Min. Volume (if applicable)

The sodium citrate collection tube must be filled to 90% of capacity.

Stability

Plasma Na Citrate
  - Frozen (preferred): 14 days
  - Room Temperature: 4 hours
  - Refrigerated: Unacceptable


Unspun Whole Blood in Na Citrate
  -Room temperature: 4 hours
  -Refrigerated: Unacceptable
  -Frozen: Unacceptable

Unacceptable Specimen Conditions

Refrigerated specimen; Quantity not sufficent; improperly labeled specimens; specimens not collected in 3.2% buffered sodium citrate tube; clotted specimens; under or over filled specimen tubes; severe hemolysis; improperly labeled specimens

Methodology

Automated

Estimated TAT

0-2 days

Testing Schedule

Monday-Saturday, & STAT

CPT Code(s)

85730
85610

Reference Range

PT 11.4 – 14.0 seconds
PTT 26 – 40 seconds

Reflex Conditions

None

Performing Lab

Incyte Diagnostics

LOINC Code(s)

5902-2, 6301-6, 14979-9